Draft genome sequence of Enterobacter cloacae ST520 harbouring blaKPC-2, blaCTX-M-15 and blaOXA-17 isolated from coastal waters of the South Atlantic Ocean

Fábio P Sellera; Miriam R Fernandes; Quézia Moura; Tiago A Souza; Louise Cerdeira; Nilton Lincopan

doi:10.1016/j.jgar.2017.07.017

Draft genome sequence of Enterobacter cloacae ST520 harbouring bla_KPC-2, bla_CTX-M-15 and bla_OXA-17 isolated from coastal waters of the South Atlantic Ocean

J Glob Antimicrob Resist. 2017 Sep:10:279-280. doi: 10.1016/j.jgar.2017.07.017. Epub 2017 Aug 4.

Authors

Fábio P Sellera¹, Miriam R Fernandes², Quézia Moura³, Tiago A Souza⁴, Louise Cerdeira², Nilton Lincopan⁵

Affiliations

¹ Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
² Department of Clinical Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil.
³ Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
⁴ Genome Investigation and Analysis Laboratory (GENIAL), Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
⁵ Department of Clinical Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. Electronic address: lincopan@usp.br.

PMID: 28827199
DOI: 10.1016/j.jgar.2017.07.017

Abstract

Objectives: Aquatic environments have contributed to the dissemination of multidrug-resistant (MDR) bacteria, representing a risk for humans and animals. The aim of this study was to report the first draft genome sequence of a MDR Enterobacter cloacae strain recovered from seawater in a public beach in Brazil.

Methods: The genome was sequenced on an Illumina MiSeq platform. De novo genome assembly was performed using SPAdes 3.10.1 and the whole genome sequence was analysed using bioinformatics tools from the Center of Genomic Epidemiology.

Results: This draft genome resulted in 5 228 857bp with 5331 protein-coding sequences, revealing the presence of bla_KPC-2, bla_CTX-M-15 and bla_OXA-17 genes, responsible for resistance to all β-lactam antibiotics. In addition, the strain was assigned to sequenced type 520 (ST520).

Conclusion: These data provide useful information for comparative genomic analysis regarding the dissemination of antibiotic resistance genes.

Keywords: Enterobacter cloacae; Seawater; Whole-genome sequencing; bla(CTX-M-15); bla(KPC-2).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Atlantic Ocean
Bacterial Proteins / genetics
Bacterial Typing Techniques
Bathing Beaches
Brazil
Drug Resistance, Multiple, Bacterial*
Enterobacter cloacae / classification*
Enterobacter cloacae / genetics
Genome, Bacterial
Multilocus Sequence Typing
Water Microbiology
Whole Genome Sequencing / methods*
beta-Lactamases / genetics*

Substances

Bacterial Proteins
beta-Lactamases