Mating-Type Determination in Schizosaccharomyces pombe

Cold Spring Harb Protoc. 2017 Aug 1;2017(8):pdb.prot091728. doi: 10.1101/pdb.prot091728.

Abstract

Here we describe how mating-type tests are conducted in Schizosaccharomyces pombe Two methods can be employed: matings with h- and h+ tester strains and polymerase chain reaction (PCR) for mat1 content.

MeSH terms

  • Genes, Mating Type, Fungal*
  • Genetics, Microbial / methods*
  • Polymerase Chain Reaction / methods*
  • Schizosaccharomyces / genetics*