An update on using CRISPR/Cas9 in the one-cell stage mouse embryo for generating complex mutant alleles

Cell Death Differ. 2017 Oct;24(10):1821-1822. doi: 10.1038/cdd.2017.122. Epub 2017 Jul 28.

Authors

Andrew J Kueh^{1

2}, Martin Pal^{1

2}, Lin Tai¹, Yang Liao^{1

2}, Gordon K Smyth^{1

3}, Wei Shi^{1

4}, Marco J Herold^{1

2}

Affiliations

¹ The Walter and Eliza Hall Institute for Medical Research, Melbourne, Victoria 3052, Australia.
² Department of Medical Biology, University of Melbourne, Melbourne, Victoria 3050, Australia.
³ Department of Mathematics and Statistics, University of Melbourne, Melbourne, Victoria 3010, Australia.
⁴ Department of Computing and Information Systems, University of Melbourne, Melbourne, Victoria 3010, Australia.

No abstract available

Publication types

Letter
Research Support, Non-U.S. Gov't

MeSH terms

Alleles*
Animals
CRISPR-Associated Protein 9 / metabolism*
CRISPR-Cas Systems / genetics*
Embryo, Mammalian / cytology*
Embryo, Mammalian / metabolism*
Gene Targeting
Mice
Mouse Embryonic Stem Cells / metabolism
Mutation / genetics*
Oligodeoxyribonucleotides / metabolism

Substances

Oligodeoxyribonucleotides
CRISPR-Associated Protein 9