Magnetic Resonance Spectroscopy (MRS)-Based Methods for Examining Cancer Metabolism in Response to Oncogenic Kinase Drug Treatment

Methods Mol Biol. 2017:1636:393-404. doi: 10.1007/978-1-4939-7154-1_25.

Abstract

Magnetic resonance spectroscopy (MRS) is an analytical technique that has been extensively used to examine reprogrammed metabolism and treatment response in cancer cells and solid tumors both in vivo and ex vivo. High-resolution MRS (HR-MRS) is one of the best methods for metabolic profiling, as it is highly quantitative, robust, and reproducible. The protocols for dual-phase extraction of cancer cells and tumors and sample preparations for high-resolution 1H and 31P HR-MRS analysis are described here. Descriptions of spectra acquisition and analysis are also included in this chapter.

Keywords: 1H-MRS; 31P-MRS; Cancer metabolism; Dual-phase extraction; Magnetic resonance spectroscopy; Nuclear magnetic resonance spectroscopy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chemical Fractionation / methods
  • Energy Metabolism / drug effects*
  • Humans
  • Lipid Metabolism
  • Magnetic Resonance Spectroscopy / methods*
  • Metabolomics* / methods
  • Oncogene Proteins / metabolism*
  • Protein Kinase Inhibitors / pharmacology*
  • Protein Kinases / metabolism*
  • Solubility

Substances

  • Oncogene Proteins
  • Protein Kinase Inhibitors
  • Protein Kinases