Nitrogen deposition has minor effect on soil extracellular enzyme activities in six Chinese forests

Abstract

Soil extracellular enzymes play a key role in mediating a range of forest ecosystem functions (i.e., carbon and nutrients cycling and biological productivity), particularly in the face of atmospheric N deposition that has been increasing at an unprecedented rate globally. However, most studies have focused only on surface soils in a single ecosystem. In this study, we aimed to determine whether the effect of simulated N deposition on the activities and ratios of soil enzymes changes with soil depth across six forest ecosystems in eastern China. We collected soil samples from three blocks×four soil depths (0-10cm, 10-20cm, 20-40cm and 40-60cm)×three N treatment levels (control, 50 and 100kgNha^-1year^-1) at each of the six forest ecosystems. We measured the activities of seven soil enzymes involved in C-, N- and P-cycling. We found that 4-5years of N addition had no significant effect on the activities and ratios of these enzymes in most cases. The interactions among N addition, site and soil depth on soil enzyme activities were not significant, except that acid phosphatase activity showed site-specific responses to N addition. Our findings suggest that the activities of soil enzymes involved in C- and N-cycling generally do not track simulated N deposition in the six forest ecosystems. Further work on plant, soil and microbial characteristics is needed to better understand the mechanisms of soil enzyme activities in response to N deposition in forest ecosystems.

Keywords: Decomposition; Forest ecosystem; Nitrogen deposition; Nutrient cycling; Soil enzyme; Stoichiometry.