Anti-arthritic activity of cell wall content of Lactobacillus plantarum in freund's adjuvant-induced arthritic rats: involvement of cellular inflammatory mediators and other biomarkers

Inflammopharmacology. 2018 Feb;26(1):171-181. doi: 10.1007/s10787-017-0370-z. Epub 2017 Jul 6.

Abstract

Alteration of microbiota is related with rheumatoid arthritis (RA) and administration of certain probiotics showed an improvement in RA. The present study was designed to find out the anti-arthritic activity of cell wall content of Lactobacillus plantarum in complete Freund's adjuvant (CFA)-induced arthritis in rats. Freund's adjuvant was injected into the left footpad in female rats on day 0 and dexamethasone (1 mg kg-1, s.c.) & cell wall content of L. plantarum (105, 107, and 109 cfu/animal, s.c.) treatment were given from day 7 to 21. The change in body weight, paw volume and arthritic index, joint stiffness, gait test, mobility test, erythrocyte sedimentation rate (ESR), serum C-reactive protein (CRP) level, serum rheumatoid factor (RF), and serum TNF-α was measured on day 21. Cell wall content of L. plantarum treated animals showed improvement in all the parameters as compared to that in CFA-treated animals and exert anti-arthritic activity.

Keywords: Arthritic index; Dexamethasone; Lactobacillus plantarum; Probiotics; Rheumatoid arthritis; Rheumatoid factor.

MeSH terms

  • Animals
  • Arthritis, Experimental / chemically induced
  • Arthritis, Experimental / drug therapy*
  • Arthritis, Experimental / metabolism
  • Biological Factors / pharmacology*
  • Biomarkers / metabolism*
  • Body Weight / drug effects
  • C-Reactive Protein / metabolism
  • Cell Wall / metabolism*
  • Female
  • Freund's Adjuvant / pharmacology
  • Inflammation Mediators / pharmacology*
  • Lactobacillus plantarum / metabolism*
  • Rats
  • Rats, Wistar
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Biological Factors
  • Biomarkers
  • Inflammation Mediators
  • Tumor Necrosis Factor-alpha
  • C-Reactive Protein
  • Freund's Adjuvant