Generation of induced pluripotent stem cells from Chinese hamster embryonic fibroblasts

Haiyun Pei; Hsu-Yuan Fu; Hiroyuki Hirai; Dong Seong Cho; Timothy D O'Brien; James Dutton; Catherine M Verfaillie; Wei-Shou Hu

doi:10.1016/j.scr.2017.04.010

Generation of induced pluripotent stem cells from Chinese hamster embryonic fibroblasts

Stem Cell Res. 2017 May:21:132-136. doi: 10.1016/j.scr.2017.04.010. Epub 2017 Apr 21.

Authors

Haiyun Pei¹, Hsu-Yuan Fu², Hiroyuki Hirai³, Dong Seong Cho⁴, Timothy D O'Brien⁵, James Dutton⁶, Catherine M Verfaillie⁷, Wei-Shou Hu⁸

Affiliations

¹ Department of Chemical Engineering and Materials Science, Minneapolis, MN, USA; Stem Cell Institute, University of Minnesota, Minneapolis, MN, USA; Stem Cell and Regenerative Medicine Lab, Beijing Institute of Transfusion Medicine, Beijing, China.
² Department of Chemical Engineering and Materials Science, Minneapolis, MN, USA.
³ Department of Cardiac Surgery, University of Michigan, Ann Arbor, MI, USA.
⁴ Department of Chemical Engineering and Materials Science, Minneapolis, MN, USA; Stem Cell Institute, University of Minnesota, Minneapolis, MN, USA.
⁵ Stem Cell Institute, University of Minnesota, Minneapolis, MN, USA; Veterinary Population Medicine Department, University of Minnesota, St. Paul, MN, USA.
⁶ Stem Cell Institute, University of Minnesota, Minneapolis, MN, USA.
⁷ Department of Development and Regeneration, Katholieke Universiteit Leuven, Leuven, Belgium.
⁸ Department of Chemical Engineering and Materials Science, Minneapolis, MN, USA; Stem Cell Institute, University of Minnesota, Minneapolis, MN, USA. Electronic address: acre@cems.umn.edu.

PMID: 28677528
DOI: 10.1016/j.scr.2017.04.010

Abstract

We derived a stable cell line from Chinese hamster embryonic fibroblasts by transduction of four mouse transcription factors (M₃O, Sox2, Klf4, and n-Myc) using a lentiviral vector. The cell line possess all the characteristics of an induced pluripotent stem cell (iPSC) line. Given that Chinese hamster ovary (CHO) cells are the predominant host cells used for therapeutic protein production and no pluripotent stem cell line or other normal cell line has been isolated from Chinese hamster, this iPSC line may serve as a useful tool for research using CHO cells or even be used for deriving new cell lines.

MeSH terms

Animals
CHO Cells
Cricetinae
Cricetulus
Induced Pluripotent Stem Cells / cytology
Induced Pluripotent Stem Cells / metabolism*
Kruppel-Like Factor 4
Lentivirus*
Mice
Transcription Factors* / biosynthesis
Transcription Factors* / genetics
Transduction, Genetic*

Substances

Klf4 protein, mouse
Kruppel-Like Factor 4
Transcription Factors

Grants and funding

P30 CA077598/CA/NCI NIH HHS/United States