Silencing of ATP11B by RNAi-Induced Changes in Neural Stem Cell Morphology

Methods Mol Biol. 2017:1622:51-61. doi: 10.1007/978-1-4939-7108-4_4.

Abstract

RNA interference (RNAi) technology is one of the main research tools in many studies of neural stem cells. This study describes effects of ATP11B on the morphology change of neural stem cells by using RNAi. ATP11B belongs to P4-ATPases family, which is preferential translocate phosphatidylserine of cell membrane. Although it exists in neural stem cells, its physiological function is poorly understood. By using RNAi technology to downregulate expression of ATP11B, we found distinct morphological changes in neural stem cells. More important, psiRNA-ATP11B-transfected cells displayed short neurite outgrowth compared to the control cells. These data strongly suggest that ATP11B plays a key role in the morphological change of neural stem cells.

Keywords: ATP11B; Morphology; Neural stem cell; RNA interference.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / genetics*
  • Adenosine Triphosphatases / metabolism
  • Cells, Cultured
  • Gene Expression Regulation, Developmental*
  • Gene Silencing*
  • Humans
  • Immunohistochemistry
  • Membrane Transport Proteins / genetics*
  • Membrane Transport Proteins / metabolism
  • Neural Stem Cells / cytology
  • Neural Stem Cells / metabolism*
  • Plasmids / genetics
  • RNA Interference*

Substances

  • Membrane Transport Proteins
  • ATP11B protein, human
  • Adenosine Triphosphatases