Effect of canine mesenchymal stromal cells loaded with paclitaxel on growth of canine glioma and human glioblastoma cell lines

A Bonomi; E Ghezzi; L Pascucci; M Aralla; V Ceserani; L Pettinari; V Coccè; A Guercio; G Alessandri; E Parati; A T Brini; O Zeira; A Pessina

doi:10.1016/j.tvjl.2017.05.005

Effect of canine mesenchymal stromal cells loaded with paclitaxel on growth of canine glioma and human glioblastoma cell lines

Vet J. 2017 May:223:41-47. doi: 10.1016/j.tvjl.2017.05.005. Epub 2017 May 19.

Authors

A Bonomi¹, E Ghezzi², L Pascucci³, M Aralla², V Ceserani⁴, L Pettinari², V Coccè¹, A Guercio⁵, G Alessandri⁴, E Parati⁴, A T Brini⁶, O Zeira², A Pessina⁷

Affiliations

¹ Department of Biomedical, Surgical and Dental Sciences, University of Milan, via Pascal 36, 20133 Milan, Italy.
² Veterinary Hospital San Michele, via I Maggio 37, 26838 Tavazzano con Villavesco, Lodi, Italy.
³ Department of Veterinary Medicine, University of Perugia, via San Costanzo 4, 06126 Perugia, Italy.
⁴ Cellular Neurobiology Laboratory, Department of Cerebrovascular Diseases, IRCCS Neurological Institute C. Besta, via Celoria 11, 20133 Milan, Italy.
⁵ Area Diagnostica Virologica dell'Istituto Zooprofilattico Sperimentale della Sicilia 'A Mirri', Palermo, Italy.
⁶ Department of Biomedical, Surgical and Dental Sciences, University of Milan, via Pascal 36, 20133 Milan, Italy; IRCCS Galeazzi Orthopaedic Institute, Milan, Italy.
⁷ Department of Biomedical, Surgical and Dental Sciences, University of Milan, via Pascal 36, 20133 Milan, Italy. Electronic address: augusto.pessina@unimi.it.

PMID: 28671070
DOI: 10.1016/j.tvjl.2017.05.005

Abstract

This study investigated whether canine mesenchymal stromal cells (cMSCs) are able to take up and release paclitaxel (PTX) in active form, and therefore whether they have potential as a tool for therapeutic delivery of this drug. cMSCs from bone marrow and adipose tissue were isolated, expanded and characterised phenotypically. cMSCs were loaded with PTX (cMSCs-PTX) and their capacity for release of PTX was determined by their effect on proliferation of cancer cells. cMSCs-PTX derived from bone marrow and adipose tissue were able to take up and then release active PTX. cMSCs-PTC inhibited proliferation of the canine glioma cell line J3T, and the human glioblastoma cell lines T98G and U87MG. The potential of canine cMSCs-PTX for treatment of canine gliomas should be investigated further.

Keywords: Canine; Drug delivery; Glioma; Mesenchymal stromal cells; Paclitaxel.

MeSH terms

Adipose Tissue / cytology
Animals
Antineoplastic Agents, Phytogenic / administration & dosage*
Bone Marrow Cells
Cell Line, Tumor
Dogs
Drug Delivery Systems
Glioblastoma / drug therapy*
Glioma / drug therapy*
Humans
Mesenchymal Stem Cells / metabolism*
Paclitaxel / administration & dosage*

Substances

Antineoplastic Agents, Phytogenic
Paclitaxel