Discovery of highly reactive peptide tag by ELISA-type screening for specific cysteine conjugation

Nobutaka Kurashige; Hirokazu Fuchida; Shigekazu Tabata; Shohei Uchinomiya; Akio Ojida

doi:10.1016/j.bmcl.2017.05.069

Discovery of highly reactive peptide tag by ELISA-type screening for specific cysteine conjugation

Bioorg Med Chem Lett. 2017 Aug 1;27(15):3486-3489. doi: 10.1016/j.bmcl.2017.05.069. Epub 2017 May 24.

Authors

Nobutaka Kurashige¹, Hirokazu Fuchida¹, Shigekazu Tabata¹, Shohei Uchinomiya¹, Akio Ojida²

Affiliations

¹ Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
² Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. Electronic address: ojida@phar.kyushu-u.ac.jp.

PMID: 28576616
DOI: 10.1016/j.bmcl.2017.05.069

Abstract

We report the discovery of a highly reactive peptide tag for the specific cysteine conjugation of proteins. Screening of cysteine-containing peptides using ELISA-type screening yielded a 19-amino acid tag (DCPPPDDAADDAADDAADD), named DCP3 tag, which enabled the rapid and selective labeling of the tag-fused protein with a synthetic zinc complex on the surface of living cells.

Keywords: ELISA; Fluorescence imaging; Peptide tag; Protein labeling.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Coordination Complexes / chemistry
Cysteine / chemistry*
Enzyme-Linked Immunosorbent Assay / methods
HEK293 Cells
Humans
Maltose-Binding Proteins / analysis
Optical Imaging* / methods
Peptides / chemistry*
Proteins / analysis*
Receptors, G-Protein-Coupled / analysis
Zinc / chemistry

Substances

Coordination Complexes
Maltose-Binding Proteins
Peptides
Proteins
Receptors, G-Protein-Coupled
Zinc
Cysteine