C1q Assay Results in Complement-Dependent Cytotoxicity Crossmatch Negative Renal Transplant Candidates with Donor-Specific Antibodies: High Specificity But Low Sensitivity When Predicting Flow Crossmatch

José M Arreola-Guerra; Luis E Morales-Buenrostro; Natalia Castelán; Adrián de Santiago; Adriana Arvizu; Norma Gonzalez-Tableros; Mayra López; Mario Vilatobá; Julio Granados; Josefina Alberú

C1q Assay Results in Complement-Dependent Cytotoxicity Crossmatch Negative Renal Transplant Candidates with Donor-Specific Antibodies: High Specificity But Low Sensitivity When Predicting Flow Crossmatch

Clin Transpl. 2015:31:285-292.

Affiliations

¹ Department of Transplantation, National Institute of Medical Sciences and Nutrition Salvador Zubirán, Mexico City, Mexico.
² Department of Nephrology-Mineral Metabolism, National Institute of Medical Sciences and Nutrition Salvador Zubirán, Mexico City, Mexico.
³ Histocompatibility Laboratory, National Institute of Medical Sciences and Nutrition Salvador Zubirán, Mexico City, Mexico.

PMID: 28514590

Abstract

In complement dependent cytotoxicity crossmatch negative renal transplant candidates with human leukocyte antigen donor-specific antibodies (DSA), both the presence of DSA C1q+ and the dominant DSA fluorescence were significantly associated with a positive flow cytometry crossmatch (FXM+). The C1q+ assay was highly specific, but had low sensitivity when predicting FXM+, so the clinical significance of a FXM+ in the absence of DSA C1q+ remains to be clarified in future studies.

Keywords: C1q; donor-specific antibodies; kidney; transplantation.