Monitoring influenza virus susceptibility to oseltamivir using a new rapid assay, iART

Euro Surveill. 2017 May 4;22(18):30529. doi: 10.2807/1560-7917.ES.2017.22.18.30529.

Abstract

A new rapid assay for detecting oseltamivir resistance in influenza virus, iART, was used to test 149 clinical specimens. Results were obtained for 132, with iART indicating 41 as 'resistant'. For these, sequence analysis found known and suspected markers of oseltamivir resistance, while no such markers were detected for the remaining 91 samples. Viruses isolated from the 41 specimens showed reduced or highly reduced inhibition by neuraminidase inhibition assay. iART may facilitate broader antiviral resistance testing.

Keywords: air-borne infections; influenza virus; surveillance; viral infections.

MeSH terms

  • Antiviral Agents / pharmacology*
  • Antiviral Agents / therapeutic use
  • Drug Resistance, Viral*
  • Humans
  • Influenza A Virus, H1N1 Subtype / drug effects*
  • Influenza A Virus, H1N1 Subtype / genetics
  • Influenza A Virus, H1N1 Subtype / isolation & purification
  • Influenza, Human
  • Microbial Sensitivity Tests / methods
  • Neuraminidase / antagonists & inhibitors*
  • Neuraminidase / genetics
  • Neuraminidase / metabolism
  • Neuraminidase / therapeutic use
  • Oseltamivir / pharmacology*
  • Oseltamivir / therapeutic use

Substances

  • Antiviral Agents
  • Oseltamivir
  • Neuraminidase