Measurements of morphological and biophysical alterations in individual neuron cells associated with early neurotoxic effects in Parkinson's disease

Su-A Yang; Jonghee Yoon; Kyoohyun Kim; YongKeun Park

doi:10.1002/cyto.a.23110

Measurements of morphological and biophysical alterations in individual neuron cells associated with early neurotoxic effects in Parkinson's disease

Cytometry A. 2017 May;91(5):510-518. doi: 10.1002/cyto.a.23110. Epub 2017 Apr 20.

Authors

Su-A Yang^{1

2}, Jonghee Yoon^{2

3}, Kyoohyun Kim^{2

3}, YongKeun Park^{2

3

4}

Affiliations

¹ Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, South Korea.
² KAIST Institute Health Science and Technology, Daejeon, 34141, South Korea.
³ Department of Physics, KAIST, Daejeon, 34141, South Korea.
⁴ Tomocube, Inc, Daejeon, 34051, South Korea.

PMID: 28426150
DOI: 10.1002/cyto.a.23110

Abstract

Parkinson's disease (PD) is a common neurodegenerative disease. However, therapeutic methods of PD are still limited due to complex pathophysiology in PD. Here, optical measurements of individual neurons from in vitro PD model using optical diffraction tomography (ODT) are presented. By measuring 3D refractive index distribution of neurons, morphological and biophysical alterations in in-vitro PD model are quantitatively investigated. It was found that neurons show apoptotic features in early PD progression. The present approach will open up new opportunities for quantitative investigation of the pathophysiology of various neurodegenerative diseases. © 2017 International Society for Advancement of Cytometry.

Keywords: 3D refractive index tomography; Parkinson's disease; cell death; label-free; quantitative phase imaging.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biophysics / methods*
Cell Count / methods
Cell Line
Humans
Neurons / pathology
Neurons / ultrastructure*
Parkinson Disease / diagnostic imaging*
Parkinson Disease / pathology