Three amino acids of a heptapeptide-induced antibody are responsible for recognition of SV40 structural proteins

Immunobiology. 1988 May;177(2):149-57. doi: 10.1016/s0171-2985(88)80035-4.

Abstract

Antibodies were elicited against the heptapeptide Asp-His-Lys-Val-Ser-Thr-Val. The sequence of the heptapeptide corresponds to amino acid residues 105-111 of Simian Virus 40 (SV40) structural protein VP2. The antipeptide antibodies recognize VP2 and amino acid sequence Asp-His-Lys (193-195) in VP1 at different degrees of denaturation. Inhibition experiments were performed with the heptapeptide as well as with the tripeptide Asp-His-Lys. Using this model system, the specificity and selectivity of peptide-induced antibodies can be demonstrated with a resolution of a single amino acid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids / immunology
  • Animals
  • Antigen-Antibody Reactions*
  • Antigens, Viral / immunology
  • Binding, Competitive
  • Electrophoresis, Polyacrylamide Gel
  • Immunization
  • Oligopeptides / chemical synthesis
  • Oligopeptides / immunology
  • Rabbits
  • Simian virus 40 / immunology*
  • Viral Proteins / immunology*
  • Viral Structural Proteins

Substances

  • Amino Acids
  • Antigens, Viral
  • Oligopeptides
  • Viral Proteins
  • Viral Structural Proteins