Multigene profiling of single circulating tumor cells

Seung-Min Park; Dawson J Wong; Chin Chun Ooi; Jared C Nesvet; Viswam S Nair; Shan X Wang; Sanjiv S Gambhir

doi:10.1080/23723556.2017.1289295

Multigene profiling of single circulating tumor cells

Mol Cell Oncol. 2017 Mar 7;4(2):e1289295. doi: 10.1080/23723556.2017.1289295. eCollection 2017.

Authors

Seung-Min Park¹, Dawson J Wong², Chin Chun Ooi³, Jared C Nesvet⁴, Viswam S Nair⁵, Shan X Wang⁶, Sanjiv S Gambhir⁷

Affiliations

¹ Department of Radiology, Stanford University School of Medicine, Stanford, CA, USA; Molecular Imaging Program at Stanford, Stanford University School of Medicine, Stanford, CA, USA.
² Department of Electrical Engineering, Stanford University , Stanford, CA, USA.
³ Department of Chemical Engineering, Stanford University , Stanford, CA, USA.
⁴ Department of Chemistry, Stanford University , Stanford, CA, USA.
⁵ Department of Radiology, Stanford University School of Medicine, Stanford, CA, USA; Division of Pulmonary and Critical Care Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA; Canary Center at Stanford for Cancer Early Detection, Stanford University School of Medicine, Palo Alto, CA, USA.
⁶ Department of Electrical Engineering, Stanford University, Stanford, CA, USA; Department of Materials Science and Engineering, Stanford University, Stanford, CA, USA; Canary Center at Stanford for Cancer Early Detection, Stanford University School of Medicine, Palo Alto, CA, USA.
⁷ Department of Radiology, Stanford University School of Medicine, Stanford, CA, USA; Molecular Imaging Program at Stanford, Stanford University School of Medicine, Stanford, CA, USA; Canary Center at Stanford for Cancer Early Detection, Stanford University School of Medicine, Palo Alto, CA, USA.

Abstract

Numerous techniques for isolating circulating tumor cells (CTCs) have been developed. Concurrently, single-cell techniques that can reveal molecular components of CTCs have become widely available. We discuss how the combination of isolation and multigene profiling of single CTCs in our platform can facilitate eventual translation to the clinic.

Keywords: Circulating tumor cells; rare-cell sorting; single-cell analysis.

Grants and funding

U54 CA199075/CA/NCI NIH HHS/United States