Microscopic diagnosis of the leaf and stem of Piper solmsianum C.DC

Microsc Res Tech. 2017 Aug;80(8):831-837. doi: 10.1002/jemt.22870. Epub 2017 Mar 30.

Abstract

Piper solmsianum C.DC., which is popularly known as pariparoba, is a shrub that measures 1-3 m in height and it inhabits areas with wet tropical soils. The objective of this study was to analyze the leaf and stem anatomy using light microscopy, scanning electron micrographs, and energy-dispersive X-ray spectroscopy in order to provide information for species identification. The anatomical profile showed the following main microscopic markers: hypostomatic leaf; hypodermis layer on both sides; pearl glands; biconvex midrib shape; five collateral vascular bundles in open arc with the central bundle larger than the others; circular stem shape; collateral vascular bundles arranged in two rings; sinuous sclerenchymatic sheath in the pith; secretory idioblasts; and starch grains in the mesophyll, in the ground parenchyma of the midrib, petiole, and in the stem; and six morphotypes of calcium oxalate crystals (styloids, cuneiform, tabular crystal rosettes, cuneiform crystal rosettes, elongated square dipyramids, as well as very elongated square dipyramids).

Keywords: anatomy; energy-dispersive X-ray spectroscopy; scanning electron microscopy.

MeSH terms

  • Microscopy, Electron, Scanning
  • Piper / chemistry
  • Piper / ultrastructure*
  • Plant Leaves / chemistry
  • Plant Leaves / ultrastructure*
  • Plant Stems / chemistry
  • Plant Stems / ultrastructure*
  • Spectrometry, X-Ray Emission