Rapid, sensitive detection of plasma IL-1 by extraction and bioassay

J Immunol Methods. 1988 Apr 6;108(1-2):33-7. doi: 10.1016/0022-1759(88)90399-7.

Abstract

A method for separating IL-1 from plasma inhibitors by silica extraction has been developed and coupled to a highly sensitive bioassay using the LBRM TG6 cell line and the I1-2 dependent HT2A cell line. Using this assay we have detected IL-1 activity in plasma from patients undergoing elective surgery.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Cell Line
  • Chromatography, Gel
  • Humans
  • Interleukin-1 / blood*
  • Interleukin-1 / isolation & purification
  • Lymphoma / metabolism*
  • Mice
  • Neoplasms, Experimental / metabolism*
  • Silicon Dioxide
  • T-Lymphocytes / metabolism*
  • Tumor Cells, Cultured

Substances

  • Interleukin-1
  • Silicon Dioxide