APCCdh1 controls cell cycle entry during liver regeneration

Ranran Cheng; Xin Liang; Quancheng Zhao; Zhusheng Lian; Lichun Tang; Chen Qiu; Huan Chen; Pumin Zhang

doi:10.1016/j.yexcr.2017.03.038

APC^Cdh1 controls cell cycle entry during liver regeneration

Exp Cell Res. 2017 May 15;354(2):78-84. doi: 10.1016/j.yexcr.2017.03.038. Epub 2017 Mar 18.

Authors

Ranran Cheng¹, Xin Liang¹, Quancheng Zhao¹, Zhusheng Lian², Lichun Tang², Chen Qiu², Huan Chen³, Pumin Zhang⁴

Affiliations

¹ Anhui Medical University, Hefei 230032, China; State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing 102206, China.
² State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing 102206, China; National Center for Protein Sciences (Beijing), Beijing 102206, China.
³ State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing 102206, China; National Center for Protein Sciences (Beijing), Beijing 102206, China. Electronic address: chenhuan@smmu.edu.cn.
⁴ Anhui Medical University, Hefei 230032, China; Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA. Electronic address: pzhang@bcm.edu.

PMID: 28322826
DOI: 10.1016/j.yexcr.2017.03.038

Abstract

Cdh1 is one of the two adaptor proteins of anaphase-promoting complex/cyclosome (APC/C), an E3 ubiquitin ligase controlling mitosis and DNA replication. To date, the in vivo functions of Cdh1 have not been fully explored. In order to characterize Cdh1 in liver regeneration, we generated a conditional knock-out mouse model. Our data showed that loss of Cdh1 leads to increased and extended S phase progression possibly due to the upregulation of cyclin D1. Moreover, the increased DNA replication resulted in activated DNA damage response. Interestingly, the final liver weight after partial hepatectomy in the Cdh1 depleted mice did not differ from that of the controls, implying that Cdh1 is not required for the competence of hepatocytes to regenerate itself.

Keywords: Cdh1; Liver regeneration; Replication.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cdh1 Proteins / metabolism*
Cell Cycle*
Cyclins / metabolism
DNA Replication
Gene Deletion
Hepatectomy
Liver Regeneration*
Mice, Knockout
Phenotype
S Phase
Stress, Physiological

Substances

Cdh1 Proteins
Cyclins

Abstract

Publication types

MeSH terms

Substances

Grants and funding