Gene transfer into hematopoietic progenitor cells mediated by an adeno-associated virus vector

Virology. 1988 Feb;162(2):483-6. doi: 10.1016/0042-6822(88)90491-6.

Abstract

We describe here the transduction of murine hematopoietic progenitor cells with the dominant selectable neomycin drug-resistance (Neo) gene using a recombinant adeno-associated virus (AAV) vector. Successful transformation of progenitor cells to drug resistance was determined to be approximately 1.5% by colony formation in the presence of geneticin sulfate (G-418). The value of AAV as an alternative to the retrovirus vector systems is discussed.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bone Marrow / physiology
  • Bone Marrow Cells
  • Cells, Cultured
  • Dependovirus / genetics*
  • Genetic Vectors*
  • Hematopoietic Stem Cells / physiology*
  • Humans
  • Transformation, Genetic*