Non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop-mediated isothermal amplification method and MinION™ nanopore sequencing

Kazuo Imai; Norihito Tarumoto; Kiyoko Amo; Makoto Takahashi; Naoya Sakamoto; Atsushi Kosaka; Yasuyuki Kato; Kei Mikita; Jun Sakai; Takashi Murakami; Yutaka Suzuki; Shigefumi Maesaki; Takuya Maeda

doi:10.1016/j.parint.2017.03.001

Non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop-mediated isothermal amplification method and MinION™ nanopore sequencing

Parasitol Int. 2018 Feb;67(1):34-37. doi: 10.1016/j.parint.2017.03.001. Epub 2017 Mar 11.

Authors

Affiliations

¹ Department of Infectious Diseases and Pulmonary Medicine, National Defense Medical College, Japan. Electronic address: kazu_imai@ndmc.ac.jp.
² Department of Infectious Disease and Infection Control, Saitama Medical University, Saitama, Japan; Center for Clinical Infectious Diseases and Research, Saitama Medical University, Saitama, Japan.
³ Department of Pediatric Emergency Medicine, Osaka City General Hospital, Osaka, Japan.
⁴ Department of Plastic Surgery, Osaka City General Hospital, Osaka, Japan.
⁵ Department of Infectious Diseases, Tokyo Metropolitan Bokutoh General Hospital, Tokyo, Japan.
⁶ Division of Preparedness and Emerging Infections, Disease Control and Prevention Center, National Center for Global Health and Medicine, Tokyo, Japan.
⁷ Department of Infectious Diseases and Pulmonary Medicine, National Defense Medical College, Japan; Department of Infectious Diseases, Keio University School of Medicine, Tokyo, Japan.
⁸ Center for Clinical Infectious Diseases and Research, Saitama Medical University, Saitama, Japan; Department of Microbiology, Saitama Medical University, Saitama, Japan.
⁹ Department of Computational Biology and Medical Science, Graduate School of Frontier Sciences, The University of Tokyo, Chiba, Japan.

PMID: 28288843
DOI: 10.1016/j.parint.2017.03.001

Abstract

Cutaneous leishmaniasis (CL) is gaining attention as a public health problem. We present two cases of CL imported from Syria and Venezuela in Japan. We diagnosed them as CL non-invasively by the direct boil loop-mediated isothermal amplification method and an innovative sequencing method using the MinION™ sequencer. This report demonstrates that our procedure could be useful for the diagnosis of CL in both clinical and epidemiological settings.

Keywords: Cutaneous leishmaniasis;; Diagnosis; LAMP;; Leishmania braziliensis; Leishmania tropica; MinION™.

Publication types

Case Reports

MeSH terms

Child, Preschool
Communicable Diseases, Imported / diagnosis*
Communicable Diseases, Imported / parasitology
Humans
Japan
Leishmania braziliensis / isolation & purification*
Leishmania tropica / isolation & purification*
Leishmaniasis, Cutaneous / diagnosis*
Leishmaniasis, Cutaneous / parasitology
Male
Middle Aged
Nanopores
Nucleic Acid Amplification Techniques / veterinary*
Sequence Analysis, DNA*
Syria
Venezuela