Three new triterpenoids isolated from the aerial parts of Ilex cornuta and protective effects against H2O2-induced myocardial cell injury

Chin J Nat Med. 2017 Feb;15(2):115-120. doi: 10.1016/S1875-5364(17)30027-4.

Abstract

In the present study, three new triterpenoids, 23-hydroxyurs-12, 18-dien-28-oic acid 3β-O-α-L-arabinopyranoside (1), 23-hydroxyurs-12, 18-dien-28-oic acid 3β-O-β-D-glucuronopyranoside-6-O-methyl ester (2), and urs-12, 18-dien-28-oic acid 3β-O-β-D-glucuronopyranoside-6-O-methyl ester (3), and a known triterpenoid, 3β-hydroxy-urs-2, 18-dien-28-oic acid (4, randialic acid B), were isolated from the aerial parts of Ilex cornuta. Their structures were identified by the spectroscopic analyses (IR, ESI-MS, HR-ESI-MS, and 1D and 2D NMR) and chemical reactions. Compound 4 showed significant cell-protective effects against H2O2-induced H9c2 cardiomyocyte injury. Compounds 1-4 did not show any significant DPPH radical scavenging activity.

Keywords: Aquifoliaceae; Cardiomyocytes Oxidative injury; Ilex cornuta; Triterpenoids.

MeSH terms

  • Animals
  • Biphenyl Compounds / metabolism
  • Cardiovascular Agents / chemistry
  • Cardiovascular Agents / isolation & purification
  • Cardiovascular Agents / pharmacology
  • Hydrogen Peroxide / metabolism
  • Ilex / chemistry*
  • Molecular Structure
  • Myocardium / cytology
  • Myocardium / pathology*
  • Myocytes, Cardiac / drug effects
  • Picrates / metabolism
  • Plant Components, Aerial / chemistry
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology*
  • Rats
  • Triterpenes / chemistry
  • Triterpenes / isolation & purification
  • Triterpenes / pharmacology*

Substances

  • Biphenyl Compounds
  • Cardiovascular Agents
  • Picrates
  • Plant Extracts
  • Triterpenes
  • randialic acid B
  • Hydrogen Peroxide
  • 1,1-diphenyl-2-picrylhydrazyl