Heat shock or Sindbis virus infection of chick embryo (CE) or baby hamster kidney (BHK) cells resulted in a decrease in the uptake of 86Rubidium+, a K+ tracer. Both stressful treatments decreased 86Rb+ uptake by inhibition of the ouabain-sensitive Na+/K+ ATPase. Alterations in the intracellular levels of monovalent ions may be involved in translational or transcriptional control of the stress response. Heat shock or Sindbis virus infection also resulted in an increase in rate of uptake of [3H]deoxy-D-glucose and a decrease in the incorporation of [3H]glucosamine or [3H]mannose into most cellular proteins. These results suggested that heat shock or Sindbis virus infection alter hexose metabolism and that abnormally glycosylated proteins may accumulate in stressed cells. Exposure of uninfected chick embryo cells to elevated temperature had little effect on the overall rate of incorporation of [32P]orthophosphate into cellular proteins. However, one protein (Mr 31,000; pp31) displayed increased incorporation of [32P]orthophosphate and two other proteins (Mr 33,000 and 20,000; pp33 and pp20) displayed decreased incorporation. Sindbis virus infection failed to mimic or to modify these heat shock induced alterations in protein phosphorylation.