Immune response of toddlers with history of prematurity

S P Muraro; P M Pitrez; A P D de Souza; B N Porto; J E Vargas; I P Ewald; J P Heinzmann-Filho; G Dos Santos; T S Baptista; T D Gandolfi; F D Machado; M H Jones; C Bonorino; R T Stein

doi:10.1016/j.aller.2016.10.020

Immune response of toddlers with history of prematurity

Allergol Immunopathol (Madr). 2017 Sep-Oct;45(5):425-431. doi: 10.1016/j.aller.2016.10.020. Epub 2017 Feb 21.

Authors

S P Muraro¹, P M Pitrez², A P D de Souza¹, B N Porto¹, J E Vargas¹, I P Ewald¹, J P Heinzmann-Filho³, G Dos Santos³, T S Baptista⁴, T D Gandolfi⁵, F D Machado⁵, M H Jones³, C Bonorino⁴, R T Stein⁶

Affiliations

¹ Laboratory of Clinical and Experimental Immunology, Brazil; Infant Center, Brazil; Institute of Biomedical Research, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Brazil.
² Laboratory of Pediatric Respirology, Brazil; Infant Center, Brazil; Institute of Biomedical Research, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Brazil.
³ Laboratory of Respiratory Physiology, Brazil; Infant Center, Brazil; Institute of Biomedical Research, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Brazil.
⁴ Laboratory of Cellular and Molecular Immunology, Brazil; Institute of Biomedical Research, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Brazil.
⁵ Laboratory of Clinical and Experimental Immunology, Brazil; Institute of Biomedical Research, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Brazil.
⁶ Laboratory of Pediatric Respirology, Brazil; Infant Center, Brazil; Institute of Biomedical Research, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Brazil. Electronic address: rstein@pucrs.br.

PMID: 28236541
DOI: 10.1016/j.aller.2016.10.020

Abstract

Background: It is not quite well established how immune responses differ in term and preterm infants beyond the first year of life. This study aimed to evaluate aspects of the innate and adaptive immune responses in a group of preterm infants in comparison with their term peers.

Methods: In this cross-sectional study peripheral blood mononuclear cells (PBMC) were isolated from preterm and term children at age three years. Innate immune response was evaluated by the analysis of TLR receptors expression on CD11c⁺HLADR^high cells and inflammatory cytokine production after PBMC stimulation with Toll like receptors (TLR) ligands. Adaptive immune response was evaluated by T cells' phenotyping and function after stimulation with polyclonal conventional T cell stimulus.

Conclusion: We have found that the patterns of innate and adaptive immune responses at 3 years of age were not affected by the fact of the children having being born preterm or at term.

Keywords: Cytokines; Immune response; Memory T cell; Preterm children; TLR expression.

MeSH terms

Adaptive Immunity
CD11c Antigen / metabolism
Child, Preschool
Cross-Sectional Studies
Cytokines / metabolism
Female
HLA-DR Antigens / metabolism
Humans
Immunity, Innate
Immunophenotyping
Infant
Infant, Premature
Inflammation Mediators / metabolism
Leukocytes, Mononuclear / immunology*
Male
Premature Birth / immunology*
T-Lymphocytes / immunology*
Toll-Like Receptors / metabolism

Substances

CD11c Antigen
Cytokines
HLA-DR Antigens
Inflammation Mediators
Toll-Like Receptors