Abstract
We transferred murine NIH 3T3 metaphase chromosomes into monkey CV-1 cells to investigate the different ouabain sensitivities of rodent and primate cells. In 16 ouabain-resistant transferents, the mouse Na,K-ATPase alpha 1 subunit gene was detected, suggesting that structural differences between the rodent and primate alpha 1 subunits determine the different ouabain sensitivities.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Cell Line
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Cells, Cultured
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Chlorocebus aethiops
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Chromosomes / physiology*
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Macromolecular Substances
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Mice
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Mice, Inbred Strains
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Ouabain / pharmacology*
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Sodium-Potassium-Exchanging ATPase / genetics*
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Transformation, Genetic
Substances
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Macromolecular Substances
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Ouabain
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Sodium-Potassium-Exchanging ATPase