Mapping and Cloning of Chemical Induced Mutations by Whole-Genome Sequencing of Bulked Segregants

Jian Hua; Shuai Wang; Qi Sun

doi:10.1007/978-1-4939-6859-6_24

Mapping and Cloning of Chemical Induced Mutations by Whole-Genome Sequencing of Bulked Segregants

Methods Mol Biol. 2017:1578:285-289. doi: 10.1007/978-1-4939-6859-6_24.

Authors

Jian Hua¹, Shuai Wang², Qi Sun³

Affiliations

¹ Plant Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, NY, 14853, USA. jh299@cornell.edu.
² Plant Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, NY, 14853, USA.
³ Biocomputing Service Unit, Institute of Biotechnology, Cornell University, Ithaca, NY, 14853, USA.

PMID: 28220434
DOI: 10.1007/978-1-4939-6859-6_24

Abstract

Here we describe a method of gene mapping and cloning of chemical induced mutations using next-generation sequencing on their backcrossed segregants. This method utilizes polymorphisms induced by chemical mutagens for mapping and therefore does not require outcrossing to a different background or a large segregating population. It can be used to clone causal mutations generated in various accessions or mutant backgrounds for dissecting complex processes such as pattern recognition receptor pathways in plant immunity.

Keywords: Cloning; Mapping; Mutagenesis; Next-generation sequencing.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Arabidopsis / genetics*
Chromosome Mapping / methods*
Cloning, Molecular / methods*
Genome, Plant / drug effects
High-Throughput Nucleotide Sequencing
Inbreeding
Mutagens / adverse effects
Mutation*
Whole Genome Sequencing

Substances

Mutagens