The Use of L-sIDOL Transgenic Mice as a Murine Model to Study Hypercholesterolemia and Atherosclerosis

Methods Mol Biol. 2017:1583:65-72. doi: 10.1007/978-1-4939-6875-6_6.

Abstract

There are many advantages to the use of mice as a model to study the regulation of cholesterol metabolism. Common models of hypercholesterolemia include low-density lipoprotein receptor deficient (LDLR -/-) mice and apolipoprotein E deficient (ApoE) -/- mice. Herein, we describe the recently generated mouse model, L-sIDOL Tg mice, which express a dominant active form of Inducible Degrader Of the Low-density lipoprotein receptor (IDOL) in a liver-specific manner. This murine model offers significant advantages over previously established models for the study of hypercholesterolemia and atherosclerosis.

Keywords: Atherosclerosis; Hypercholesterolemia; IDOL; LDL cholesterol; LDLR; Liver; Mouse model; Transgenic.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apolipoproteins E / deficiency
  • Atherosclerosis* / genetics
  • Atherosclerosis* / metabolism
  • Atherosclerosis* / pathology
  • Disease Models, Animal
  • Hypercholesterolemia* / genetics
  • Hypercholesterolemia* / metabolism
  • Hypercholesterolemia* / pathology
  • Liver* / metabolism
  • Liver* / pathology
  • Mice
  • Mice, Knockout
  • Organ Specificity / genetics
  • Receptors, LDL / deficiency
  • Ubiquitin-Protein Ligases* / biosynthesis
  • Ubiquitin-Protein Ligases* / genetics

Substances

  • Apolipoproteins E
  • Receptors, LDL
  • Mylip protein, mouse
  • Ubiquitin-Protein Ligases