KDM2A integrates DNA and histone modification signals through a CXXC/PHD module and direct interaction with HP1

Nucleic Acids Res. 2017 Feb 17;45(3):1114-1129. doi: 10.1093/nar/gkw979.

Abstract

Functional genomic elements are marked by characteristic DNA and histone modification signatures. How combinatorial chromatin modification states are recognized by epigenetic reader proteins and how this is linked to their biological function is largely unknown. Here we provide a detailed molecular analysis of chromatin recognition by the lysine demethylase KDM2A. Using biochemical approaches we identify a nucleosome interaction module within KDM2A consisting of a CXXC type zinc finger, a PHD domain and a newly identified Heterochromatin Protein 1 (HP1) interaction motif that mediates direct binding between KDM2A and HP1. This nucleosome interaction module enables KDM2A to decode nucleosomal H3K9me3 modification in addition to CpG methylation signals. The multivalent engagement with DNA and HP1 results in a nucleosome binding circuit in which KDM2A can be recruited to H3K9me3-modified chromatin through HP1, and HP1 can be recruited to unmodified chromatin by KDM2A. A KDM2A mutant deficient in HP1-binding is inactive in an in vivo overexpression assay in zebrafish embryos demonstrating that the HP1 interaction is essential for KDM2A function. Our results reveal a complex regulation of chromatin binding for both KDM2A and HP1 that is modulated by DNA- and H3K9-methylation, and suggest a direct role for KDM2A in chromatin silencing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Cell Line
  • Chromobox Protein Homolog 5
  • Chromosomal Proteins, Non-Histone / chemistry*
  • Chromosomal Proteins, Non-Histone / genetics
  • Chromosomal Proteins, Non-Histone / metabolism*
  • CpG Islands
  • Cricetinae
  • DNA Methylation
  • F-Box Proteins / chemistry*
  • F-Box Proteins / genetics
  • F-Box Proteins / metabolism*
  • HEK293 Cells
  • HeLa Cells
  • Histones / metabolism
  • Humans
  • Jumonji Domain-Containing Histone Demethylases / chemistry*
  • Jumonji Domain-Containing Histone Demethylases / genetics
  • Jumonji Domain-Containing Histone Demethylases / metabolism*
  • Models, Genetic
  • Mutation
  • Neoplasms / genetics
  • Neoplasms / metabolism
  • Nucleosomes / genetics
  • Nucleosomes / metabolism
  • Protein Interaction Domains and Motifs
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Two-Hybrid System Techniques
  • Zebrafish
  • Zinc Fingers

Substances

  • Chromosomal Proteins, Non-Histone
  • F-Box Proteins
  • Histones
  • Nucleosomes
  • Recombinant Fusion Proteins
  • Chromobox Protein Homolog 5
  • Jumonji Domain-Containing Histone Demethylases
  • KDM2A protein, human