Fluorogenic boronate-based probe-lactulose complex for full-aqueous analysis of peroxynitrite

Kai-Bin Li; Lei Dong; Siqi Zhang; Wei Shi; Wen-Ping Jia; De-Man Han

doi:10.1016/j.talanta.2017.01.028

Fluorogenic boronate-based probe-lactulose complex for full-aqueous analysis of peroxynitrite

Talanta. 2017 Apr 1:165:593-597. doi: 10.1016/j.talanta.2017.01.028. Epub 2017 Jan 7.

Authors

Kai-Bin Li¹, Lei Dong², Siqi Zhang³, Wei Shi³, Wen-Ping Jia³, De-Man Han⁴

Affiliations

¹ Department of Chemistry, Taizhou University, Jiaojiang 318000, PR China. Electronic address: lkbecust@126.com.
² Institut de Chimie et Biochimie Moléculaires et Supramoléculaires,, Laboratoire de Chimie Organique 2 - Glycochimie, UMR 5246, Université Lyon 1 and CNRS, 43 Boulevard du 11 Novembre 1918, F-69622, Villeurbanne, France.
³ Department of Chemistry, Taizhou University, Jiaojiang 318000, PR China.
⁴ Department of Chemistry, Taizhou University, Jiaojiang 318000, PR China. Electronic address: hdm@tzc.edu.cn.

PMID: 28153303
DOI: 10.1016/j.talanta.2017.01.028

Abstract

A selective fluorogenic boronate-based probe-lactulose complex was evaluated for the rapid analysis of peroxynitrite (ONOO^-) based on a reaction-based indicator displacement assay (RIA). The probe was synthesised by a simple nucleophilic substitution reaction between a boronic acid moiety and a well known laser dye, DCM. Fluorescence analyses showed that the probe had an off-on response to lactulose, forming a fluorogenic probe-lactulose complex. The subsequent addition of ONOO^- selectively quenched the fluorescence of the complex over other Reactive Oxygen/ Nitrogen Species (ROS/RNS) tested. The complex can be applied for the rapid determination of ONOO^- in full aqueous solution with good linear range, and has also proven suitable for monitoring ONOO^- in living cells and real water samples.

Keywords: DCM; Fluorescent probe; Full aqueous; Lactulose; Peroxynitrite.