Investigation of IGF2, IGFBP2 and p63 proteins in rhabdomyosarcoma tumors

Yongbo Yu; Libing Fu; Shen Wang; Yaqiong Jin; Shujing Han; Ping Chu; Jie Lu; Yongli Guo; Lejian He; Xin Ni

doi:10.1016/j.ghir.2017.01.002

Investigation of IGF2, IGFBP2 and p63 proteins in rhabdomyosarcoma tumors

Growth Horm IGF Res. 2017 Apr:33:17-22. doi: 10.1016/j.ghir.2017.01.002. Epub 2017 Jan 13.

Authors

Yongbo Yu¹, Libing Fu¹, Shen Wang², Yaqiong Jin¹, Shujing Han¹, Ping Chu¹, Jie Lu¹, Yongli Guo¹, Lejian He³, Xin Ni⁴

Affiliations

¹ Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck Surgery, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing, China.
² Clinical Diagnostic Center, 302nd Hospital of the People's Liberation Army, Beijing, China.
³ Department of Pathology, Beijing Children's Hospital, Capital Medical University, Beijing, China. Electronic address: lejianhe@sina.com.
⁴ Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck Surgery, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing, China; Department of Otolaryngology, Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, Beijing, China. Electronic address: nixin@bch.com.cn.

PMID: 28129571
DOI: 10.1016/j.ghir.2017.01.002

Abstract

Many efforts have been made to address involvement of the insulin-like growth-factor (IGF) pathway in rhabdomyosarcoma (RMS) pathogenesis, but the actual role of IGF in RMS is still controversial.

Objective: To investigate the implications of IGF2, IGFBP2 and p63 in RMS, and further explored their potential interaction.

Design: A total of 114 specimens of RMS along with clinic-pathologic characteristics were collected from the year of 2003 to 2013. Protein abundance was detected by immunohistochemical staining, potential relationships between protein levels and clinic-pathological parameters were applied using correlation analysis.

Results: The results showed positive correlation between IGFBP2 and p63 (r=0.271, p=0.003), suggesting that the interaction of IGFBP2 and p63 might account for the pathogenesis of RMS. In the subtype analysis, positive correlation was still found in embryonal rhabdomyosarcoma (ERMS, r=0.214, p=0.034) and alveolar rhabdomyosarcoma (ARMS, r=0.498, p=0.048). By focusing on the interaction of IGF pathway and p63, our results reveal additional signs to elucidate difference of pathogenesis and severity between ERMS and ARMS.

Conclusions: The present study provides novel evidence to elucidate RMS pathogenesis and may be beneficial to clinical diagnosis and therapy for RMS.

Keywords: Insulin-like growth-factor; Rhabdomyosarcoma; p63.

MeSH terms

Adolescent
Adult
Biomarkers, Tumor / metabolism*
Child
Child, Preschool
Female
Humans
Infant
Infant, Newborn
Insulin-Like Growth Factor Binding Protein 2 / metabolism*
Insulin-Like Growth Factor II / metabolism*
Male
Prognosis
Rhabdomyosarcoma, Alveolar / metabolism*
Rhabdomyosarcoma, Alveolar / pathology
Rhabdomyosarcoma, Embryonal / metabolism*
Rhabdomyosarcoma, Embryonal / pathology
Transcription Factors / metabolism*
Tumor Suppressor Proteins / metabolism*
Young Adult

Substances

Biomarkers, Tumor
IGF2 protein, human
IGFBP2 protein, human
Insulin-Like Growth Factor Binding Protein 2
TP63 protein, human
Transcription Factors
Tumor Suppressor Proteins
Insulin-Like Growth Factor II