Abstract
In the field of dendritic cell based genetic immunization, previously we showed that liposomes of cationic amphiphiles containing mannose-mimicking shikimoyl headgroup are promising DNA vaccine carriers for dendritic cell (DC) transfection. The present structure-activity study reports on the influence of spacer length (between mannose-mimicking headgroups and quaternary nitrogen centers) in modulating the DC-transfection efficiencies. Further, we report on the anti-melanoma immune response inducing properties of the promising cationic amphiphiles in syngeneic C57BL/6J mice under prophylactic settings.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cancer Vaccines / administration & dosage*
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Cancer Vaccines / immunology
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Cancer Vaccines / therapeutic use
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Cell Line, Tumor
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Dendritic Cells / immunology*
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Dendritic Cells / metabolism
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Drug Carriers / chemistry*
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Female
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Hydrophobic and Hydrophilic Interactions
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Male
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Mannose / analogs & derivatives*
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Melanoma / immunology
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Melanoma / prevention & control*
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Mice, Inbred C57BL
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Surface-Active Agents / chemistry
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Transfection / methods*
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Vaccination / methods
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Vaccines, DNA / administration & dosage*
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Vaccines, DNA / immunology
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Vaccines, DNA / therapeutic use
Substances
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Cancer Vaccines
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Drug Carriers
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Surface-Active Agents
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Vaccines, DNA
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Mannose