The lack of standardised protocols for the assessment of functional antibodies has hindered Streptococcus pyogenes research and the development of vaccines. A robust, high throughput opsonophagocytic bactericidal assay to determine protective antibodies in human and rabbit serum has been developed that utilises bioluminescence as a rapid read out.
Keywords: Bactericidal assay; Bioluminescence; Correlates of protection; Group A Streptococcus (GAS); Lancefield assay; Protective antibodies.
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