Reclassification of non-type strain Clostridium pasteurianum NRRL B-598 as Clostridium beijerinckii NRRL B-598

Karel Sedlar; Jan Kolek; Ivo Provaznik; Petra Patakova

doi:10.1016/j.jbiotec.2017.01.003

Reclassification of non-type strain Clostridium pasteurianum NRRL B-598 as Clostridium beijerinckii NRRL B-598

J Biotechnol. 2017 Feb 20:244:1-3. doi: 10.1016/j.jbiotec.2017.01.003. Epub 2017 Jan 19.

Authors

Karel Sedlar¹, Jan Kolek², Ivo Provaznik³, Petra Patakova⁴

Affiliations

¹ Department of Biomedical Engineering, Brno University of Technology, Technicka 12, 616 00 Brno, Czechia. Electronic address: sedlar@feec.vutbr.cz.
² Department of Biotechnology, University of Chemistry and Technology Prague, Technicka 5, 166 28 Prague, Czechia. Electronic address: jan.kolek@vscht.cz.
³ Department of Biomedical Engineering, Brno University of Technology, Technicka 12, 616 00 Brno, Czechia. Electronic address: provaznik@feec.vutbr.cz.
⁴ Department of Biotechnology, University of Chemistry and Technology Prague, Technicka 5, 166 28 Prague, Czechia. Electronic address: petra.patakova@vscht.cz.

PMID: 28111164
DOI: 10.1016/j.jbiotec.2017.01.003

Abstract

The complete genome sequence of non-type strain Clostridium pasteurianum NRRL B-598 was introduced last year; it is an oxygen tolerant, spore-forming, mesophilic heterofermentative bacterium with high hydrogen production and acetone-butanol fermentation ability. The basic genome statistics have shown its similarity to C. beijerinckii rather than the C. pasteurianum species. Here, we present a comparative analysis of the strain with several other complete clostridial genome sequences. Besides a 16S rRNA gene sequence comparison, digital DNA-DNA hybridization (dDDH) and phylogenomic analysis confirmed an inaccuracy of the taxonomic status of strain Clostridium pasteurianum NRRL B-598. Therefore, we suggest its reclassification to be Clostridium beijerinckii NRRL B-598. This is a specific strain and is not identical to other C. beijerinckii strains. This misclassification explains its unexpected behavior, different from other C. pasteurianum strains; it also permits better understanding of the bacterium for a future genetic manipulation that might increase its biofuel production potential.

Keywords: Clostridium beijerinckii; Clostridium pasteurianum; Phylogenomic analysis; Reclassification.

Publication types

Comparative Study

MeSH terms

Clostridium / genetics*
Clostridium beijerinckii / classification*
Clostridium beijerinckii / genetics
DNA, Bacterial / genetics
DNA, Ribosomal / genetics
Genome, Bacterial
Phylogeny
RNA, Ribosomal, 16S / genetics*
Sequence Analysis, DNA / methods*

Substances

DNA, Bacterial
DNA, Ribosomal
RNA, Ribosomal, 16S