CD3+CD56+ natural killer T cell activity in children with different forms of juvenile idiopathic arthritis and the influence of etanercept treatment on polyarticular subgroup

Clin Immunol. 2017 Mar:176:1-11. doi: 10.1016/j.clim.2016.12.001. Epub 2016 Dec 24.

Abstract

Juvenile idiopathic arthritis (JIA) has three major onset types with widely varying clinical features. We assessed the natural killer T (NKT) cell function in patients with different JIA subtypes, and found systemic patients exhibited lower NKT cell counts, perforin and granzyme B expression, while the pauciarticular and polyarticular patients displayed higher perforin and granzyme B expression as compared with the controls. The synovial fluid had more NKT cells with higher levels of perforin, granzyme B, and tumour necrosis factor (TNF)-α than peripheral cells. The polyarticular patients that responded to etanercept had lower NKT cell counts, intracellular perforin, granzyme B and the mean fluorescence intensity of TNF-α than the patients that did not respond. Treatment with etanercept reduced the granzyme B and perforin, interferon (IFN)-γ and TNF-α expression in NKT cells in the responsive group. Therefore, a higher NKT cell function may indicate a decreased response to etanercept in polyarticular patients.

Keywords: Etanercept; Juvenile idiopathic arthritis; Natural killer T cell.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arthritis, Juvenile / drug therapy*
  • Arthritis, Juvenile / immunology*
  • CD3 Complex / immunology*
  • Cell Count / methods
  • Child
  • Child, Preschool
  • Etanercept / therapeutic use*
  • Female
  • Granzymes / immunology
  • Humans
  • Interferon-gamma / immunology
  • Killer Cells, Natural / drug effects
  • Killer Cells, Natural / immunology
  • Lymphocyte Activation / drug effects
  • Lymphocyte Activation / immunology
  • Male
  • Natural Killer T-Cells / drug effects
  • Natural Killer T-Cells / immunology*
  • Perforin / immunology
  • Synovial Fluid / drug effects
  • Synovial Fluid / immunology
  • Tumor Necrosis Factor-alpha / immunology

Substances

  • CD3 Complex
  • Tumor Necrosis Factor-alpha
  • Perforin
  • Interferon-gamma
  • Granzymes
  • Etanercept