Use of the APTIMA Combo 2 Assay and a Secondary Algorithm to Detect and Confirm Chlamydia trachomatis in Rectal-Only Infections

Kanti Pabbaraju; Sallene Wong; Kara Gill; Alberto Severini; Felicia Roy; Jennifer Gratrix; Ameeta E Singh; Prenilla Naidu; Ron Read; Steven J Drews

doi:10.1097/OLQ.0000000000000552

Use of the APTIMA Combo 2 Assay and a Secondary Algorithm to Detect and Confirm Chlamydia trachomatis in Rectal-Only Infections

Sex Transm Dis. 2017 Feb;44(2):118-119. doi: 10.1097/OLQ.0000000000000552.

Authors

Kanti Pabbaraju¹, Sallene Wong, Kara Gill, Alberto Severini, Felicia Roy, Jennifer Gratrix, Ameeta E Singh, Prenilla Naidu, Ron Read, Steven J Drews

Affiliation

¹ From the *Provincial Laboratory for Public Health, Calgary, Alberta; †National Microbiology Laboratory, Public Health Agency of Canada; ‡Department of Medical Microbiology, Faculty of Health Sciences, University of Manitoba; §STI Centralized Services, Alberta Health Services; ¶Department of Medicine, University of Alberta; ∥Department of Medicine, **Department of Microbiology, Immunology and Infectious Diseases, University of Calgary; ††Provincial Laboratory for Public Health, Edmonton; and ‡‡Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada.

PMID: 27984551
DOI: 10.1097/OLQ.0000000000000552

Abstract

We sought to confirm the results of 81 rectal specimens positive for Chlamydia trachomatis by the APTIMA Combo 2 assay among patients with concurrently collected negative genitourinary specimens. A total of 79 (97.5%) samples were confirmed by the APTIMA single target assay and/or sequencing of the C. trachomatis ompA gene.

MeSH terms

Algorithms
Chlamydia Infections / diagnosis*
Chlamydia Infections / microbiology
Chlamydia trachomatis / genetics
Chlamydia trachomatis / isolation & purification*
Female
Humans
Male
Rectum / microbiology*