Characterization of iPSCs derived from dermal fibroblasts from a healthy 19year old female

Stem Cell Res. 2016 Nov;17(3):597-599. doi: 10.1016/j.scr.2016.10.002. Epub 2016 Oct 19.

Abstract

Primary fibroblasts from a healthy 19years old female were reprogrammed by transduction of retroviruses OCT4, SOX2, c-MYC and KLF4. iPSCs were characterized by immunocytochemistry, embryonic body-formation, DNA-fingerprint and karyotype analysis and comparative transcriptome analyses with the human embryonic stem cell line H1 revealed a Pearsons correlation coefficient of 0.8952.

MeSH terms

  • Cell Differentiation
  • Cell Line
  • Cellular Reprogramming
  • Comparative Genomic Hybridization
  • DNA Fingerprinting
  • Dermis / cytology
  • Embryoid Bodies / cytology
  • Embryoid Bodies / metabolism
  • Female
  • Fibroblasts / cytology
  • Genetic Vectors / genetics
  • Genetic Vectors / metabolism
  • Healthy Volunteers
  • Human Embryonic Stem Cells / cytology
  • Human Embryonic Stem Cells / metabolism
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Induced Pluripotent Stem Cells / metabolism
  • Karyotype
  • Kruppel-Like Factor 4
  • Microscopy, Fluorescence
  • Retroviridae / genetics
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Young Adult

Substances

  • KLF4 protein, human
  • Kruppel-Like Factor 4
  • Transcription Factors