Matrilin-2 regulates proliferation, apoptosis and cell cycle during radiation-induced injury in HPAEpiC cell

Junming Luo; Menglan Zhang; Hong Huang; Yichun Wang; Xin Yuan; Siqing Ma; Jingshi Liu; Shiying Zhou; Shukun Zhang

doi:10.1016/j.bbrc.2016.12.022

Matrilin-2 regulates proliferation, apoptosis and cell cycle during radiation-induced injury in HPAEpiC cell

Biochem Biophys Res Commun. 2017 Apr 8;485(3):577-583. doi: 10.1016/j.bbrc.2016.12.022. Epub 2016 Dec 5.

Authors

Junming Luo¹, Menglan Zhang¹, Hong Huang¹, Yichun Wang², Xin Yuan³, Siqing Ma⁴, Jingshi Liu², Shiying Zhou¹, Shukun Zhang⁵

Affiliations

¹ Department of Pathology, Qinghai People's Provincial Hospital, Xining 810007, Qinghai Province, People's Republic of China.
² Department of Anesthesiology, Hunan Provincial Tumor Hospital, Central South University, Changsha 410013, Hunan Province, People's Republic of China.
³ Department of Internal Medicine, Qinghai People's Provincial Hospital, Xining 810007, Qinghai Province, People's Republic of China.
⁴ Department of Critical Care Medicine, Qinghai People's Provincial Hospital, Xining 810007, Qinghai Province, People's Republic of China.
⁵ Department of Pathology, Qinghai People's Provincial Hospital, Xining 810007, Qinghai Province, People's Republic of China. Electronic address: zsk0475@163.com.

PMID: 27923659
DOI: 10.1016/j.bbrc.2016.12.022

Abstract

Radiation pulmonary injury is related to the accumulation of extracellular matrix proteins in the alveolar interstitial space. Matrilin-2 as a component of extracellular filamentous networks, present higher level in the lung tissue from irradiated mice and irradiated pulmonary epithelial cell line, HPAEpiC cells. Knockdown of endogenous matrilin-2 prevents the apoptosis of HPAEpiC cell induced by the irradiation injury. Consistently, over-expression of matrilin-2 reduced the proliferation and induced apoptosis of HPAEpiC cells. Matrilin-2 promotes the expression of p21 via increasing the transcriptional activity of p53, by which induces the G1 phase arresting in HPAEpiC cells. In summary, matrilin-2, increased by irradiation, reduced the proliferation and induces apoptosis of pulmonary epithelial cells via p53/p21 pathway.

Keywords: Apoptosis; Cell cycle; Matrilin-2; Proliferation; Radiation pulmonary injury.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Apoptosis / genetics*
Apoptosis / radiation effects
Blotting, Western
Cell Proliferation / genetics*
Cell Proliferation / radiation effects
Cells, Cultured
Cyclin-Dependent Kinase Inhibitor p21 / genetics
Cyclin-Dependent Kinase Inhibitor p21 / metabolism
Epithelial Cells / metabolism*
Epithelial Cells / radiation effects
G1 Phase Cell Cycle Checkpoints / genetics*
G1 Phase Cell Cycle Checkpoints / radiation effects
Gene Expression / radiation effects
Humans
Lung / metabolism
Lung / radiation effects
Male
Matrilin Proteins / genetics*
Matrilin Proteins / metabolism
Mice, Inbred C57BL
Pulmonary Alveoli / cytology
RNA Interference
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction / genetics
Signal Transduction / radiation effects
Tumor Suppressor Protein p53 / genetics
Tumor Suppressor Protein p53 / metabolism

Substances

Cyclin-Dependent Kinase Inhibitor p21
Matrilin Proteins
Tumor Suppressor Protein p53