Plant extracts: initial screening, identification of bioactive compounds and effect against Candida albicans biofilms

Future Microbiol. 2017 Jan:12:15-27. doi: 10.2217/fmb-2016-0094. Epub 2016 Dec 6.

Abstract

Aim: This study screened plants from Brazilian Pantanal for Candida albicans antibiofilm activity.

Material & methods: Sixty extracts were obtained from ten plants using different extraction methods. Antifungal activity was assessed. Effects on biofilm inhibition and disruption and cytotoxicity were also evaluated. The most active extract was chemically characterized.

Results: Buchenavia tomentosa ethanolic extract showed noticeable antifungal activity and was selected for biofilm experiments. Subinhibitory concentration of extract inhibited fungal adhesion. Maximum killing reached 90% of C. albicans cells in suspension and 65% of cells in biofilms. The active extract was noncytotoxic. Chemical characterization showed the presence of phenols. Ellagic and gallic acids showed activity on C. albicans.

Conclusion: B. tomentosa extract and its isolated compound, ellagic acid, presented antibiofilm activity and low toxicity.

Keywords: Candida albicans; antifungal agents; bioactive compounds; biofilms.

MeSH terms

  • Antifungal Agents / pharmacology*
  • Biofilms / drug effects*
  • Brazil
  • Caco-2 Cells
  • Candida albicans / drug effects*
  • Combretaceae / chemistry
  • Ellagic Acid / pharmacology
  • Gallic Acid / pharmacology
  • Humans
  • Phenols / pharmacology
  • Plant Extracts / pharmacology*

Substances

  • Antifungal Agents
  • Phenols
  • Plant Extracts
  • Ellagic Acid
  • Gallic Acid