The cloned human oestrogen receptor contains a mutation which alters its hormone binding properties

EMBO J. 1989 Jul;8(7):1981-6. doi: 10.1002/j.1460-2075.1989.tb03604.x.

Abstract

We demonstrate here that the human oestrogen receptor (hER) cDNA clone pOR8 obtained from MCF-7 cells contains an artefactual point mutation which results in the substitution of a valine for a glycine at amino acid position 400 (Gly-400----Val-400). This mutation in the hormone binding domain of the cloned hER destabilizes its structure and decreases its apparent affinity for oestradiol at 25 degrees C, but not at 4 degrees C, when compared with the wild-type hER with a Gly-400.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Breast Neoplasms
  • Cell Line
  • Cloning, Molecular*
  • DNA, Neoplasm / genetics
  • DNA-Directed DNA Polymerase
  • Female
  • Gene Amplification
  • Genes
  • Glycine
  • Humans
  • Kinetics
  • Molecular Sequence Data
  • Mutation*
  • Oligonucleotide Probes
  • Receptors, Estrogen / genetics*
  • Receptors, Estrogen / metabolism
  • Valine

Substances

  • DNA, Neoplasm
  • Oligonucleotide Probes
  • Receptors, Estrogen
  • DNA-Directed DNA Polymerase
  • Valine
  • Glycine