Potentiometric Aptasensing of Vibrio alginolyticus Based on DNA Nanostructure-Modified Magnetic Beads

Guangtao Zhao; Jiawang Ding; Han Yu; Tanji Yin; Wei Qin

doi:10.3390/s16122052

Potentiometric Aptasensing of Vibrio alginolyticus Based on DNA Nanostructure-Modified Magnetic Beads

Sensors (Basel). 2016 Dec 2;16(12):2052. doi: 10.3390/s16122052.

Authors

Guangtao Zhao^{1

2}, Jiawang Ding³, Han Yu^{4

5}, Tanji Yin⁶, Wei Qin⁷

Affiliations

¹ Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS); Shandong Provincial Key Laboratory of Coastal Environmental Processes, YICCAS, Yantai 264003, Shandong, China. gtzhao@yic.ac.cn.
² University of Chinese Academy of Sciences, Beijing 100049, China. gtzhao@yic.ac.cn.
³ Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS); Shandong Provincial Key Laboratory of Coastal Environmental Processes, YICCAS, Yantai 264003, Shandong, China. jwding@yic.ac.cn.
⁴ Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS); Shandong Provincial Key Laboratory of Coastal Environmental Processes, YICCAS, Yantai 264003, Shandong, China. hyu@yic.ac.cn.
⁵ University of Chinese Academy of Sciences, Beijing 100049, China. hyu@yic.ac.cn.
⁶ Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS); Shandong Provincial Key Laboratory of Coastal Environmental Processes, YICCAS, Yantai 264003, Shandong, China. tjyin@yic.ac.cn.
⁷ Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS); Shandong Provincial Key Laboratory of Coastal Environmental Processes, YICCAS, Yantai 264003, Shandong, China. wqin@yic.ac.cn.

Abstract

A potentiometric aptasensing assay that couples the DNA nanostructure-modified magnetic beads with a solid-contact polycation-sensitive membrane electrode for the detection of Vibrio alginolyticus is herein described. The DNA nanostructure-modified magnetic beads are used for amplification of the potential response and elimination of the interfering effect from a complex sample matrix. The solid-contact polycation-sensitive membrane electrode using protamine as an indicator is employed to chronopotentiometrically detect the change in the charge or DNA concentration on the magnetic beads, which is induced by the interaction between Vibrio alginolyticus and the aptamer on the DNA nanostructures. The present potentiometric aptasensing method shows a linear range of 10-100 CFU mL^-1 with a detection limit of 10 CFU mL^-1, and a good specificity for the detection of Vibrio alginolyticus. This proposed strategy can be used for the detection of other microorganisms by changing the aptamers in the DNA nanostructures.

Keywords: DNA nanostructures; Vibrio alginolyticus; magnetic beads; potentiometric aptasensing; protamine.

MeSH terms

Aptamers, Nucleotide / chemistry
Biosensing Techniques / methods*
DNA / chemistry*
Nanostructures / chemistry
Potentiometry / methods*
Vibrio alginolyticus / physiology*

Substances

Aptamers, Nucleotide
DNA