Rapid assessment of tetanus vaccine-induced immunity in Bangladesh and the Gambia

Diagn Microbiol Infect Dis. 2017 Mar;87(3):272-274. doi: 10.1016/j.diagmicrobio.2016.11.011. Epub 2016 Nov 24.

Abstract

We have developed recombinant fragment C based rapid point of care dipstick devices to assess tetanus immunization status using plasma or whole blood. The devices demonstrated specificity of 0.90 and sensitivity of 0.90 (whole blood)/0.94 (plasma) at field sites in Bangladesh and The Gambia when compared to a commercial ELISA with the immune cut-off titer set as ≥0.1IU/mL.

Keywords: Dipstick; Fragment C; Lateral flow; Tetanus; Vaccine.

MeSH terms

  • Adult
  • Antibodies, Bacterial / blood*
  • Bangladesh
  • Child
  • Gambia
  • Humans
  • Male
  • Peptide Fragments / genetics
  • Peptide Fragments / immunology*
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology*
  • Sensitivity and Specificity
  • Tetanus / immunology*
  • Tetanus Toxin / genetics
  • Tetanus Toxin / immunology*
  • Tetanus Toxoid / immunology*
  • Vaccination

Substances

  • Antibodies, Bacterial
  • Peptide Fragments
  • Recombinant Proteins
  • Tetanus Toxin
  • Tetanus Toxoid
  • tetanus toxin fragment C