Evaluation of interleukin 8 +2767 A/T polymorphism in visceral leishmaniasis

Alireza Ahmadi; Mehrdad Hajilooi; Ghasem Solgi; Mohammad Abasi; Ahad Bazmani; Alireza Khalilian; Mohammad Matini; Khosro Sardarian

doi:10.1016/j.apjtm.2016.09.010

Evaluation of interleukin 8 +2767 A/T polymorphism in visceral leishmaniasis

Asian Pac J Trop Med. 2016 Nov;9(11):1075-1077. doi: 10.1016/j.apjtm.2016.09.010. Epub 2016 Nov 1.

Authors

Alireza Ahmadi¹, Mehrdad Hajilooi², Ghasem Solgi², Mohammad Abasi¹, Ahad Bazmani², Alireza Khalilian¹, Mohammad Matini¹, Khosro Sardarian³

Affiliations

¹ Department of Internal Medicine, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
² Department of Immunology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Islamic Republic of Iran.
³ Department of Internal Medicine, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. Electronic address: khsardarian@gmail.com.

PMID: 27890367
DOI: 10.1016/j.apjtm.2016.09.010

Abstract

Objective: To evaluated the relationship between the genetic variations at IL-8 +2767 position with VL pathogenesis among Iranian patients.

Methods: Three groups including patients with VL clinical presentation and leishmania seropositive (n = 124), patients seropositive but without clinical presentation (n = 82) and healthy controls (n = 63) were selected to conduct this cross-sectional study. Polymorphism at +2767 position of IL-8 was investigated using PCR-RFLP techniques. Anti-leishmania antibody titration was evaluated by the immunoflorescence technique.

Results: We observed higher significant frequencies +2767 A/A and A/T genotypes in Group 1 compared to Group 2 and healthy controls (P = 0.001). Also, patients in Group 1 carrying A/A genotype showed higher titer of anti-leishmania antibody than patients with A/T and T/T genotypes (P = 0.05). The validity of the data was analyzed using Hardy-Weinberg equilibrium and one way analysis of variance (ANOVA), as well as χ² tests.

Conclusions: Our findings indicate that the IL-8 +2767 polymorphism is significantly involved in impaired immune responses against VL and it could be considered as a risk factor for the VL progress.

Keywords: Interleukin 8; Polymorphism; Visceral leishmaniasis.