Calcium-dependent chloride transient currents in the immature oocyte of the frog, Rana esculenta

Arch Ital Biol. 1989 Mar;127(2):69-80.

Abstract

Transient currents of chloride were studied in the plasma membrane of immature frog oocyte in voltage clamp conditions. The transients appeared to be activated by an influx of Ca2+ from the external medium. The mechanism leading to a surge of intracellular Ca2+ concentration needed at least 30 sec before full recovery. It was inhibited by substituting Ba2+ for Ca2+ in the external medium, or in the presence of La3+, Co2+ and Cd2+, or when external Na+ was replaced by Li+. Verapamil proved ineffective. The data suggest that an intracellular system of Ca-activated Ca-release is present in the frog oocyte, which can be primarily activated by membrane hyperpolarization via an influx of Ca2+ through non-selective channels.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium / pharmacology
  • Calcium / physiology*
  • Chlorides / physiology*
  • Electric Stimulation
  • Membrane Potentials
  • Oocytes / physiology*
  • Rana esculenta / physiology*

Substances

  • Chlorides
  • Calcium