Oxidative damage to myelin proteins accompanies peripheral nerve motor dysfunction in aging C57BL/6 male mice

J Neurol Sci. 2016 Nov 15:370:47-52. doi: 10.1016/j.jns.2016.09.021. Epub 2016 Sep 13.

Abstract

Aging is associated with a decline in peripheral nerve function of both motor and sensory nerves. The decline in function of peripheral sensorimotor nerves with aging has been linked to sarcopenia, the age-related decline in muscle mass and function that significantly compromises the quality of life in older humans. In this study, we report a significant increase in oxidized fatty acids and insoluble protein carbonyls in sciatic nerves of aged C57BL/6 male mice (28-30mo) that exhibit a profound decline in motor nerve function and degenerative changes in both axon and myelin structure, compared to young mice (6-8mo). Our data further suggests that this age-related loss of function of peripheral motor nerves is likely precipitated by changes in mechanisms that protect and/or repair oxidative damage. We predict that interventions that target these mechanisms may protect against age-related decline in peripheral sensorimotor nerve function and likely improve the debilitating outcome of sarcopenia in older humans.

Keywords: Aging; Carbonylation; Lipoxygenase; Oxidative stress; Peripheral nerves.

MeSH terms

  • Aging / pathology*
  • Aging / physiology*
  • Animals
  • Chromatography, High Pressure Liquid
  • Fatty Acids / metabolism*
  • Immunoblotting
  • Male
  • Mice, Inbred C57BL
  • Neural Conduction / physiology
  • Oxidation-Reduction
  • Proteasome Endopeptidase Complex / metabolism
  • Protein Carbonylation / physiology*
  • Sciatic Nerve / pathology*
  • Sciatic Nerve / physiopathology*
  • Spectrometry, Mass, Electrospray Ionization
  • Tandem Mass Spectrometry

Substances

  • Fatty Acids
  • Proteasome Endopeptidase Complex