Encapsulation of the HDACi Ex527 into Liposomes and Polymer-Based Particles

Methods Mol Biol. 2017:1510:387-398. doi: 10.1007/978-1-4939-6527-4_29.

Abstract

Incorporation of drugs into particles can improve their therapeutic effectiveness. Solubility, half-life time, targeting, and the release of the drug can be modified by the encapsulation into a particle. Histone deacetylase inhibitors have a great potential to be used as therapeutics for many different diseases. In this chapter, we describe the inclusion of the low molar mass HDACi Ex527 into polymer-based particles and liposomes.

Keywords: Acetalated dextran; Cellular uptake; Ex527; Histone deacetylase inhibitor; Liposomes; PLGA.

MeSH terms

  • Acetylation
  • Carbazoles / chemistry
  • Carbazoles / pharmacology*
  • Cell Survival / drug effects
  • Dextrans / chemistry
  • Drug Compounding / methods*
  • Flow Cytometry
  • Fluorescent Dyes / chemistry
  • HEK293 Cells
  • Half-Life
  • Histone Deacetylase Inhibitors / chemistry
  • Histone Deacetylase Inhibitors / pharmacology*
  • Humans
  • Lactic Acid / chemistry
  • Liposomes / chemistry
  • Liposomes / metabolism*
  • Phosphatidylcholines / chemistry
  • Polyglycolic Acid / chemistry
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Rhodamines / chemistry
  • Solubility

Substances

  • 6-chloro-2,3,4,9-tetrahydro-1H-carbazole-1-carboxamide
  • Carbazoles
  • Dextrans
  • Fluorescent Dyes
  • Histone Deacetylase Inhibitors
  • Liposomes
  • Phosphatidylcholines
  • Rhodamines
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Polyglycolic Acid
  • Lactic Acid
  • rhodamine B