Evaluation of Different Blood Collection Tubes and Blood Storage Conditions for the Preservation and Stability of Cell-Free Circulating DNA for the Analysis of the Methylated mSEPT9 Colorectal Cancer Screening Marker

Adv Exp Med Biol. 2016:924:175-178. doi: 10.1007/978-3-319-42044-8_32.

Abstract

For the subsequent analysis of the methylated mSEPT9 colorectal cancer screening marker in plasma, different blood collection tubes and blood storage conditions were investigated. The study demonstrated that methylated Septin 9 (mSEPT9) can be consistently detected in plasma samples derived from whole blood samples collected with S-Monovette® K3E and BD Vacutainer ® K2EDTA tubes stored at 2-8 °C for a maximum of 24 h and for samples collected in S-Monovette CPDA tubes stored at 18-25 °C for up to 48 h.

Keywords: Blood; Collection tube; Colorectal cancer; Epigenetic marker; Septin 9; Stability.

Publication types

  • Evaluation Study

MeSH terms

  • Adult
  • Aged
  • Biomarkers, Tumor / blood
  • Biomarkers, Tumor / genetics
  • Blood Preservation / methods*
  • Blood Specimen Collection / instrumentation
  • Blood Specimen Collection / methods*
  • Colorectal Neoplasms / blood
  • Colorectal Neoplasms / diagnosis
  • Colorectal Neoplasms / genetics*
  • DNA Methylation
  • DNA, Neoplasm / blood
  • DNA, Neoplasm / genetics*
  • Edetic Acid / chemistry
  • Female
  • HeLa Cells
  • Humans
  • Male
  • Middle Aged
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Septins / genetics*
  • Temperature
  • Time Factors

Substances

  • Biomarkers, Tumor
  • DNA, Neoplasm
  • Edetic Acid
  • SEPTIN9 protein, human
  • Septins