Site specific protein O-glucosylation with bacterial toxins

Y Sun; L M Willis; H R Batchelder; M Nitz

doi:10.1039/c6cc06223g

Site specific protein O-glucosylation with bacterial toxins

Chem Commun (Camb). 2016 Oct 27;52(88):13024-13026. doi: 10.1039/c6cc06223g.

Authors

Y Sun¹, L M Willis¹, H R Batchelder¹, M Nitz¹

Affiliation

¹ Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, Ontario M5S 3H6, Canada.

PMID: 27748773
DOI: 10.1039/c6cc06223g

Abstract

Using a MALDI-MS based assay, the kinetic parameters for peptide glucosylation using the C. difficile toxin B glycosyltransferase domain were determined. The minimum consensus sequence for glucosylation was YXXTXFXXY and the optimal peptide found was YAPTVFDAY. Using this sequence, homogenous glucosylated proteins could be readily produced.

MeSH terms

Bacterial Proteins / metabolism*
Bacterial Toxins / metabolism*
Clostridioides difficile / growth & development
Clostridioides difficile / metabolism*
Glycosylation
Glycosyltransferases / metabolism*
Peptide Fragments / metabolism*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

Bacterial Proteins
Bacterial Toxins
Peptide Fragments
Glycosyltransferases