GABA- and glycine-containing neuronal elements were visualized in the rat spinal cord by immunohistochemistry employing antisera raised against conjugated GABA and glycine. GABA- and glycine-like immunoreactivities were localized in axon terminals and neuronal perikarya. GABA- and glycine-like immunoreactive terminals were in synaptic contact with perikarya and dendrites of most spinal neurons. Single spinal motoneurons appeared to be in contact with GABA- or glycine-like immunoreactive terminals. These findings suggest that these neurons are regulated postsynaptically by both GABA and glycine.