Expression of Sialyl Lewis a, Sialyl Lewis x, Lewis y, Gal-3, Gal-7, STMN1 and p16 in cervical dysplasia

Future Oncol. 2017 Jan;13(2):145-157. doi: 10.2217/fon-2016-0259. Epub 2016 Sep 20.

Abstract

Aim: Cervical intraepithelial neoplasia (CIN) is commonly divided into three grades. Guidelines increasingly recommend surgery only in CIN 3 lesions. We investigated markers to evaluate differences in CIN 2 and 3 lesions as well as possible predictors for regression/progression in CIN 2 lesions.

Materials & methods: Biopsies (n = 128) of healthy cervical tissue and CIN 1-3 were stained for Sialyl Lewis a, Sialyl Lewis x, Lewis y, Gal-3, Gal-7, STMN1 and p16.

Results: We observed significant differences between CIN 2 and 3 lesions for Sialyl Lewis a, Sialyl Lewis x, Gal-3, Gal-7, STMN1 and p16. Expression of Sialyl Lewis a was significantly higher in CIN 2 patients who progressed during follow-up.

Conclusion: Significant differences in marker expression support the differentiation of CIN 2 and 3. Lewis a may help to predict progression/regression in CIN 2 patients.

Keywords: Gal-3; Gal-7; HPV; STMN1; Sialyl Lewis a; Sialyl Lewis x and Lewis y; cervical intraepithelial neoplasia; conization; p16.

MeSH terms

  • Biomarkers, Tumor*
  • CA-19-9 Antigen
  • Case-Control Studies
  • Cyclin-Dependent Kinase Inhibitor p16 / genetics
  • Cyclin-Dependent Kinase Inhibitor p16 / metabolism*
  • Disease Progression
  • Female
  • Gene Expression
  • Humans
  • Immunohistochemistry
  • Neoplasm Grading
  • Oligosaccharides / metabolism*
  • Sialyl Lewis X Antigen
  • Stathmin / genetics
  • Stathmin / metabolism*
  • Uterine Cervical Dysplasia / genetics
  • Uterine Cervical Dysplasia / metabolism*
  • Uterine Cervical Dysplasia / pathology*

Substances

  • Biomarkers, Tumor
  • CA-19-9 Antigen
  • Cyclin-Dependent Kinase Inhibitor p16
  • Oligosaccharides
  • STMN1 protein, human
  • Sialyl Lewis X Antigen
  • Stathmin