MicroRNA-1284 inhibits proliferation and induces apoptosis in SGC-7901 human gastric cancer cells

Biotechnol Lett. 2017 Jan;39(1):33-38. doi: 10.1007/s10529-016-2213-1. Epub 2016 Sep 15.

Abstract

Objectives: To explore the functional effects of miR-1284 on gastric cancer cells.

Results: Overexpression of miR-1284 significantly reduced SGC-7901 cell proliferation, but improved apoptosis. However, miR-1284 suppression displayed the inversed impacts. Furthermore, the protein levels of p27, Bax, procaspase-3 and active caspase-3 were up-regulated by miR-1284 overexpression, but were down-regulated by miR-1284 suppression. The level of Bcl-2 was down-regulated by miR-1284 overexpression, while it was up-regulated by miR-1284 suppression. The level of p21 was unaffected.

Conclusion: These results suggest that miR-1284 overexpression might be a suppressor for gastric cancer via controlling of cell proliferation and apoptosis.

Keywords: Apoptosis; Cell proliferation; Gastric cancer; SGC-7901 cells; miR-1284.

MeSH terms

  • Apoptosis / genetics
  • Apoptosis / physiology*
  • Caspase 3 / metabolism
  • Cell Line, Tumor
  • Cell Proliferation / genetics
  • Cell Proliferation / physiology*
  • Gene Expression Regulation, Neoplastic / genetics
  • Gene Expression Regulation, Neoplastic / physiology
  • Humans
  • MicroRNAs / genetics
  • MicroRNAs / physiology*
  • Stomach Neoplasms / genetics*
  • Stomach Neoplasms / metabolism

Substances

  • MIRN1284 microRNA, human
  • MicroRNAs
  • Caspase 3