Abstract
We have developed a two-photon fluorescent tracer (Pyr-affibody) that shows high selectivity for human epidermal growth factor receptor-2 (HER-2). Pyr-affibody showed absorption and emission maxima at 439 and 574 nm, respectively, with a two-photon absorption cross-section value of 40 × 10-50 cm4s/photon (GM) at 750 nm in aqueous buffer solution. The effective two-photon action cross-section value measured in HeLa cells was 600 GM at 730 nm, a value sufficient to obtain bright two-photon microscopy (TPM) images. Using Pyr-affibody, it was possible to detect HER-2 overexpressing cells and breast cancers at a depth of 90-130 μm in live mouse tissue by TPM.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Benzofurans / chemical synthesis
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Benzofurans / pharmacology*
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Benzofurans / radiation effects
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Breast Neoplasms / diagnostic imaging*
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Cell Line, Tumor
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Fluorescent Dyes / chemical synthesis
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Fluorescent Dyes / pharmacology*
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Fluorescent Dyes / radiation effects
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Humans
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Light
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Mice, Inbred BALB C
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Pyrazines / chemical synthesis
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Pyrazines / pharmacology*
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Pyrazines / radiation effects
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Receptor, ErbB-2 / metabolism*
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Recombinant Fusion Proteins / chemical synthesis
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Recombinant Fusion Proteins / pharmacology*
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Recombinant Fusion Proteins / radiation effects
Substances
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Benzofurans
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Fluorescent Dyes
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Pyrazines
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Recombinant Fusion Proteins
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ERBB2 protein, human
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Receptor, ErbB-2